Composite
NemA

Part:BBa_K1398003:Design

Designed by: Exeter iGEM 2014   Group: iGEM14_Exeter   (2014-08-11)


NemA (Inducible Construct)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 746
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was created to allow for the over-expression of NemA, so it contains high expression regulatory sequences. A lactose inducible promoter has been used to give control over expression.


Source

The original source of NemA was E. coli. The regulatory sequences were acquired from the iGEM repository.

References